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Life Science

​Dieckmann can provide custom synthesis and CRO service of various bio-chemistry compounds from mg to kg level for some clients’ bioscience research. We are committed to researching and developing these compounds with high synthesis technology, such as: bio-inhibitors, antibody adjuvants for antibody preparation,fluorescent probe molecular, and so on.
1921-70-6 2,6,10,14-四甲基十五烷
2,6,10,14-Tetramethylpentadecane
Pristane

M.F: C19H40
M.W: 268.53

>98%
Pristane is used as protocols for antibody production.is used to precondition the peritoneal cavity of mice, prior to the induction of ascites fluid with myeloma cells. Pristane is used to induce plasmacytomas in mice as models of human myltiple myeloma.It induces a disease similar to lupus erythematosus in mice, making it useful for autoimmune studies. Pristane also induces arthritis in rats as a rodent model for the study of rheumatoid arthritis.
  MDV3100 Enzalutamide (MDV3100) is an androgen-receptor (AR)antagonist with IC50 of 36 nM in LNCaP cells
1268524-70-4 (+)JQ1 (+)-JQ1 is a BET bromodomain inhibitor, with IC50 of 77 nM/33 nM for BRD4(1/2) in cell-free assays, binding to all bromodomains of the BET family, but not to bromodomains outside the BET family.
763113-22-0 Olaparib Olaparib (AZD2281, KU0059436) is a selective inhibitor of PARP1/2 with IC50 of 5 nM/1 nM in cell-free assays, 300-times less effective against tankyrase-1. Phase 3.
  EPZ-643 8 EPZ-6438 is a potent, and selective EZH2 inhibitor with Kiand IC50 of 2.5 nM and 11 nM in cell-free assays, exhibiting a 35-fold selectivity versus EZH1 and >4,500-fold selectivity relative to 14 other HMTs.
35013-72-0 D-生物素-N-羟基琥珀酰亚胺酯
N-Succinimidyl D-Biotinate

MF:C14H19N3O5S
MW:341.38
Purity:>98%
Biotin NHS is an amino reactive biotin reagent used in the preparation of biotinylated surfaces or polypeptides. It's the biological dyes and indicator。
66612-29-1 N-(4-氨丁基)-N-乙基异鲁米诺
ABEI

M.F: C14H20N4O2
M.W: 276.34
>98%
ABEI,Chemiluminescence reagent, efficient luminescence NH2 - coupling agent, used to detect a wide variety of proteins, and with the ability of picomole detection range, and has obvious advantage, compare with the traditional radiation immunity analysis method .
3682-14-2 异鲁米诺
Isoluminol

M.F: C8H7N3O2
M.W:177.1600
>98%
Chemiluminescence reagent
76384-55-9 MPEI
1-methyl-3-[(E)-2-(1-methylpyridin-1-ium-4-yl)ethenyl]indole iodideI

M.F: C17H19IN2
M.W:376.2348
>98%
MPEI™ Green-Fluorescent cyanine dye (Product No.) is a cell-permeant nucleic acid stain that can selectively stain rRNA in the nucleoli. Although virtually nonfluorescent in the absence of nucleic acids, MPEI™ exhibits bright green fluorescence when bound to rRNA (absorption/emission maxima ~458/540 nm), but only weak fluorescence when bound to ct-DNA (Figure 1). Eukaryotic cells stained with the MPEI™ dye show a staining pattern consistent with that of an RNA-selective probe (SYTO® RNASelect™ stain). Maximal fluorescence is observed in the nucleoli, with faint fluorescence found throughout the cytoplasm. The RNA localization of the MPEI™ dye is further supported by RNase and DNase treatments: a) Control treatment; b) upon treatment with DNase, there is no significant loss of fluorescence; c) upon treatment with RNase, the nucleolar and cytoplasmic intensities are significantly reduced, as compared with control cells; Because the MPEI™ dye is cell permeant, it is suitable for counterstaining use in live cells. For more detail information, please refer to (ACS Chem. Biol. 2015, 10, 1171−1175)


Figure 1. Absorption and emission spectrum of MPEI without or with calf thymus DNA (ct-DNA) or 16S and 32S rRNA in T.E. buffer at pH 7.5.


Figure 2. The fluorometric titration of MPEI with the addition of rRNA in T.E. buffer. The RNA localization of the MPEI™ dye is further supported by RNase and DNase treatments.
Key words:
ortho-Phthalaldehyde;

OPA derivatives;

Lys-labeling;

Lysine-labeling;

Lys-specific bioconjugation (PBS,PH7.2);

Amine-specific bioconjugation (PBS,PH7.2)
 
3-(1,3-dimethoxy-1,3-dihydroisobenzofuran-5-yl)propanoic acid
MF: C13H16O5
Molecular Weight: 252.26


ortho-Phthalaldehyde (OPA) and its derivatives are found to react chemoselectively with amino groups on peptides and proteins rapidly and tracelessly under the physiological condition via formation of phthalimidines, which provides a novel and promising approach when performing bioconjugation on native proteins. The notable advantages of this method over the existing native protein lysine-labeling approaches include a traceless process, a self-reacting, specific and fast reaction, ease of operation, and the ability to use nonhydrolyzable reagents. Its applications have been effectively demonstrated including conjugation of peptides and proteins, and generation of an active PEGlyated L-asparaginase.
Reference Literature:Traceless and Chemoselective Amine Bioconjugation via Phthalimidine Formation in Native Protein Modification Chun Ling Tung, Clarence T. T. Wong, Eva Yi Man Fung, and Xuech
pent-4-yn-1-yl 3-(3,4-diformylphenyl)propanoate
MF: C16H16O4
MW: 272.30
OPA-PRA-PEG (PRA-propionic acid


MF: 5K
146368-16-3 Cy3-NHS ester

Appearance:Red Powder
M.F:C34H40ClN3O4
M.W:590.15
Cy3 NHS ester is a kind of reactive dye used for labeling amino of bio-molecular , including dissolved proteins, peptides, oligonucleotides and DNA. For sensitive protein,may choose Cy3 NHS ester with water-soluble sulfonic acid group, without any co-solvent.
146368-14-1 Cy5-NHS ester
Appearance:Dark blue
powder
M.W:616.19
M.F:C36H42ClN3O4
Cy5 is a very common fluorophores used in the field of diagnostics and life science research with the maximum emission in the red zone, many CCD detector has maximum sensitivity.
Cy5 NHS ester is a kind of reactive dye used for labeling proteins, peptides, oligonucleotides.